human nl20  (ATCC)

Journal: Food Technology and Biotechnology

Article Title: Comparative Cytotoxicity Evaluation of Heat-Assisted vs Cold Water Extractions of Six Medicinal Fungi against Breast and Lung Cancer Cells

doi: 10.17113/ftb.62.02.24.8424

Figure Lengend Snippet: Half maximal inhibitory concentration (IC 50 ) of Inonotus obliquus cold water extract and its protein and non-protein fractions against A549 lung carcinoma and non-tumorigenic NL20 cells after 72 h of treatment

Article Snippet: Nontumorigenic human lung epithelial cell line NL20 (CRL-2503, ATCC) was cultured in Ham’s F-12 medium (Nacalai Tesque Inc.) supplemented with φ =10 % FBS.

Techniques: Concentration Assay

Journal: Food Technology and Biotechnology

Article Title: Comparative Cytotoxicity Evaluation of Heat-Assisted vs Cold Water Extractions of Six Medicinal Fungi against Breast and Lung Cancer Cells

doi: 10.17113/ftb.62.02.24.8424

Figure Lengend Snippet: Growth inhibition of A549 lung carcinoma (full line) and NL20 non-tumorigenic (dotted line) cell lines after 72 h of treatment in the MTT assay with the cold water extract of I. obliquus (green) and its protein (blue) and non-protein (red) fractions

Article Snippet: Nontumorigenic human lung epithelial cell line NL20 (CRL-2503, ATCC) was cultured in Ham’s F-12 medium (Nacalai Tesque Inc.) supplemented with φ =10 % FBS.

Techniques: Inhibition, MTT Assay

Journal: Biomedicines

Article Title: Repurposing Niclosamide as a Novel Anti-SARS-CoV-2 Drug by Restricting Entry Protein CD147

doi: 10.3390/biomedicines11072019

Figure Lengend Snippet: HuR binds BSG mRNA and regulates its expression. ( a ) CD147 expression in COVID-19 patients’ blood samples. Here, 6 out of 19 early patients, 6 out of 36 middle patients, and 0 out of 22 late patients are severely ill (the rest are considered as mild/moderate). ( b ) Western blotting analysis of HuR and CD147 protein in HuR knock-out clones of MDA-MB-231 cells with or without niclosamide treatment. Tubulin was used as the loading control. NIC, niclosamide. ( b’ ) Quantified relative level of CD147 HG and LG in ( b ). ( c ) Western blotting analysis of HuR and CD147 protein in doxycycline-inducible HuR TET-off system in Hela cells. Dox, doxycycline. ( c’ ) Quantified relative level of CD147 and HuR in ( c ). ( d – f ) RNP-IP analysis of relative enrichment of BSG transcripts in HuR-immunoprecipitation in ( d ) H460, ( e ) NL20, and ( f ) WI-38 cells. ( g ) Western blotting of HuR protein in the pull-down complex by BSG RNA probes in NL20 and WI-38 cells. *** p < 0.001; ** 0.001 < p < 0.01; * 0.01 < p < 0.05. NS, not significant. Two-tailed T -test was applied for statistics.

Article Snippet: Human breast cancer cell line MDA-MB-231, colon carcinoma cell line RKO, lung cancer cell line H460, human embryonal kidney (HEK) 293-FT cells, human fibroblast cell line WI-38, human lung bronchus epithelial cell line NL20, and mouse lung cancer cell line Lewis lung-2 (LL/2) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Western Blot, Knock-Out, Clone Assay, Control, Immunoprecipitation, Two Tailed Test

Journal: Biomedicines

Article Title: Repurposing Niclosamide as a Novel Anti-SARS-CoV-2 Drug by Restricting Entry Protein CD147

doi: 10.3390/biomedicines11072019

Figure Lengend Snippet: Immunocytochemistry and immunohistochemistry staining for HuR and the effect of niclosamide on HuR translocation. ( a – d’ ) Immunofluorescence staining for the detection of HuR and the effect of niclosamide on HuR cytoplasmic translocation. WI-38 ( a – b’ ) and NL20 ( c – d’ ) cells were exposed to niclosamide or DMSO for 48 h. HuR is presented in green. Nuclei were counterstained with DAPI (blue). ( e , e’ ) Immunohistochemistry staining for HuR protein in the LL/2 tumor tissue with ( e ) PBS or ( e’ ) niclosamide treatment for 5 days. Arrows point to the cytoplasmic HuR.

Article Snippet: Human breast cancer cell line MDA-MB-231, colon carcinoma cell line RKO, lung cancer cell line H460, human embryonal kidney (HEK) 293-FT cells, human fibroblast cell line WI-38, human lung bronchus epithelial cell line NL20, and mouse lung cancer cell line Lewis lung-2 (LL/2) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Immunocytochemistry, Immunohistochemistry, Staining, Translocation Assay, Immunofluorescence

Journal: Biomedicines

Article Title: Repurposing Niclosamide as a Novel Anti-SARS-CoV-2 Drug by Restricting Entry Protein CD147

doi: 10.3390/biomedicines11072019

Figure Lengend Snippet: Niclosamide inhibits HuR nucleocytoplasmic translocation. ( a – f ) Western blot of HuR and CD147 in nuclear and cytoplasmic fractions with niclosamide treatment at different concentrations and different times in NL20 ( a , b ), H460 ( c , d ), and WI-38 ( e , f ). Quantified relative levels of cytoplasmic HuR in NL20 (as in blue box), H460 (as in red box), and WI-38 (as in green box) are shown in ( a’ – f’ ), respectively. ( g ) Western blot of HuR and CD147 protein in Hela, 293FT, RKO, and MDA-MB-231 cells treated with niclosamide at different concentrations. NIC, niclosamide.

Article Snippet: Human breast cancer cell line MDA-MB-231, colon carcinoma cell line RKO, lung cancer cell line H460, human embryonal kidney (HEK) 293-FT cells, human fibroblast cell line WI-38, human lung bronchus epithelial cell line NL20, and mouse lung cancer cell line Lewis lung-2 (LL/2) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Translocation Assay, Western Blot